Services
Genome editing of cell lines
End-to-end solutions to generate gene-edited cell lines. The editing services include but are not limited to, knockout, knock-in of fluorescent tags, epitope tags, or any sequence of choice. Creation or correction of point mutations. precise deletion of sequences from the genome, like promoters, miRNA sequences etc. Deleting large DNA/chromosome fragments (>100 kbs), inversion of parts of chromosomes, translocation of chromosomal arms etc.
DNA Construct
Design and construction of complex DNA constructs up to 25 Kb with multiple inserts.
Gene Knockdown
shRNA design and shRNA viruses ready for transfection. siRNA pools against genes of interest for transient knockdown.
Immortalization
Immortalisation of primary cell lines using various methods.
Stable cell line
Generating stable expression cell lines.
Inducible cell line
Doxycycline/ other Inducible overexpression cell lines generation.
Library
cDNA library construction.
Additional Services
a) Mycoplasma testing and PCR-DIY kit.
b) Cell line authentication services for Human, mouse, dog, and rat cell lines.
c) Primary cell lines from mouse.
d) Primary cell lines from Rat.