

Our Technology
Gene Editing:
Our gene editing technology integrates various genome manipulation tools to achieve precise modifications in cells or organisms. Utilizing advanced gene editing techniques, we can delete, insert, replace, or rearrange DNA within the genome. CRISPR has become the tool of choice due to its simple design and versatile applications.
Before the advent of CRISPR, genome engineering faced limitations in most mammalian cells, excluding mouse embryonic stem cells. CRISPR has revolutionized this landscape, enabling genome engineering in all mammalian cells and organisms. Initially employed for creating double-strand breaks and gene knockouts, CRISPR's precision, when combined with other genetic manipulation tools, has expanded possibilities. At V-Edit, we bring extensive experience in synergizing CRISPR with other genetic techniques to engineer mammalian genomes.
Whether you aim to knockout, edit, or correct genes, insert or delete specific sequences, or undertake more complex manipulations like creating translocations, V-Edit provides end-to-end solutions. Our in-house Gibson assembly pipeline empowers us to efficiently create complex DNA constructs for precise gene editing. Integrated with highly efficient transfection methods and intuitive screening assays, our gene editing strategies are customized for each project, ensuring not only high efficiency but also easy detection of the desired editing events.
The comprehensive gene editing strategy employed by V-Edit makes our service unique, efficient, reliable, and adds significant value to your gene editing projects.

Design and construction of DNA constructs:
DNA constructs of varying complexity are essential components of gene editing. At V-Edit, we've developed an in-house DNA assembly design pipeline to guarantee the creation of the most suitable DNA constructs for each project. Leveraging our expertise in Gibson assembly and related tools, we seamlessly employ cloning to build these DNA constructs. These constructs, utilized in the form of plasmids or single-strand DNA, are combined with CRISPR for the precise engineering of genomes.
